The Spaniards will open in Group D against Japan.
De Gea was left off the Euro team in an attempt to be rested well from club duties and be ready for the Olympics, while Mata, who scored a goal at the Euros was given limited minutes.
On Thursday, the pair alongside the likes of Javi Martinez, Jordi Alba, Koke, Adrian and the exciting Jordi Alba - have been given the task of getting the group favourites off to a winning start.
Expected to play as well is the exciting winger Iker Munain, who is on the bench with Oriol Romeu and Cristian Tello.
Spain v Japan Teams
Spain Team 1. David De Gea 3. Alvaro Dominguez 4. Javi Martinez 5. Inigo Martinez 6. Jordi Alba 12. Martin Montoya 11. Koke 15. Isco 7. Adrian 9. Rodrigo 10. Juan Mata.
Subs: 18. Diego Marino 2. Cesar Azpilicueta 8. Iker Munain 13. Alberto Botia 14. Oriol Romeu 16. Cristian Tello 17. Ander Herrera.
Japan: 1. Shuichi Gonda 2. Yuhei Tokunaga 4. Hiroki Sakai 5. Maya Yoshida 13. Daisuke Suzuki 3. Takahiro Ogihara 10. Keigo Higashi 16. Hotaru Yamaguchi 17. Hiroshi Kiyotake 7. Yuki Otsu 11. Kensuke Nagai.
Subs: 18. Shunsuke Ando 6. Taisuke Muramatsu 8. Kazuya Yamamura 9. Kenyu Sugimoto 12. Gotuko Sakai 14. Takashi Usami 15. Manabu Saito
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